DNA methylation is recognized as a promising biomarker for many different cancers, although its current clinical use is somewhat limited. Through my research, I hope to develop a protein-based methylation detection assay that can be used to accurately detect methylation of gene promoters in fixed cells rather than purified DNA.
Brooke E. Tam, Yining Hao, Hadley D. Sikes. “An examination of critical parameters in hybridization-based epigenotyping using magnetic microparticles,” Biotechnology Progress, 2018. DOI: 10.1002/btpr.2644.
Brandon W. Heimer, Brooke Tam, Alissa F. Minkovsky and Hadley D. Sikes. “Using nanobiotechnology to increase the prevalence of epigenotyping assays in precision medicine,” WIREs Nanomedicine & Nanobiotechnology, 2017, 9 (1). DOI: 10.1002/wnan.1407.
Brooke E. Tam, Ki-Joo Sung and Hadley D. Sikes. “Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA,”Molecular Systems Design and Engineering, 2016, 1: 273-277. DOI: 10.1039/C6ME00073H.
Brandon W. Heimer, Brooke E. Tam and Hadley D. Sikes. “Characterization and Directed Evolution of a Methyl Binding Domain Protein for High-Sensitivity DNA Methylation Analysis,” Protein Engineering, Design and Selection, 2015. DOI:10.1093/protein/gzv046.