Epigenetic Publications

 


Brooke E. Tam, Yining Hao, Hadley D. Sikes. “An examination of critical parameters in hybridization-based epigenotyping using magnetic microparticles,” Biotechnology Progress, 2018, in press.


 

Brooke E. Tam, Ki-Joo Sung and Hadley D. Sikes. “Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA,”Molecular Systems Design and Engineering, 2016, 1: 273-277. DOI: 10.1039/C6ME00073H.

 

 

 


 

Brandon W. Heimer, Brooke Tam, Alissa F. Minkovsky and Hadley D. Sikes. “Using nanobiotechnology to increase the prevalence of epigenotyping assays in precision medicine,” WIREs Nanomedicine & Nanobiotechnology, 2017, 9 (1). DOI: 10.1002/wnan.1407.

 

 


BWH PEDS pic

 

 

Brandon W. Heimer, Brooke E. Tam and Hadley D. Sikes. “Characterization and Directed Evolution of a Methyl Binding Domain Protein for High-Sensitivity DNA Methylation Analysis,Protein Engineering, Design and Selection, 2015. DOI: 10.1093/protein/gzv046.

 

 


evaluating the sensitivity epigenotyping

 

B.W. Heimer, T.A. Shatova, J.K. Lee, K. Kaastrup and H.D. Sikes. “Evaluating the Sensitivity of Hybridization-Based Epigenotyping using a Methyl Binding Domain Protein,” Analyst, 2014, 139 (15): 3695-3701. DOI:10.1039/C4AN00667D. (Cover Story)

 

 

 


Functional heterologous

 

M.E. Boyd, B.W. Heimer, H.D. Sikes. “Functional heterologous expression and purification of a mammalian methyl-CpG binding domain in suitable yield for DNA methylation profiling assays,” Protein Expression & Purification, 2012, 82 (2): 332-338. DOI:10.1016/j.pep.2012.01.016.