Peer-Reviewed Publications


Ki Joo Sung, Eric A. Miller and Hadley D. Sikes, submitted.

Justin M. Paloni, Eric A. Miller, Hadley D. Sikes, and Bradley D. Olsen, in revision.

Kassi T. Stein and Hadley D. Sikes, in revision.

Seunghyeon Kim and Hadley D. Sikes. “Phenolphthalein-conjugated hydrogel formation under visible light irradiation for reducing variability of colorimetric biodetection,” ACS Applied Bio Materials, 2018. DOI: 10.1021/acsabm.8b00148

James F. Powers, Brent Cochran, James D. Baleja, Hadley D. Sikes, Xue Zhang, Inna Lomakin, Troy F. Langford, Kassi T. Stein, Arthur S. Tischler. “A unique model for SDH- deficient GIST: an endocrine related cancer,” Endocrine-Related Cancer, 2018, in press.

Troy F. Langford, Beijing K. Huang, Joseph B. Lim, Sun Jin Moon, Hadley D. Sikes. “Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe,” Nature Communications, 2018, in press.

Eric A. Miller, Yara Jabbour Al Maalouf, Hadley D. Sikes. Design principles for enhancing sensitivity in paper-based diagnostics via large-volume processing,” Analytical Chemistry, 2018. DOI: 10.1021/acs.analchem.8b02113.

Brooke E. Tam, Yining Hao, Hadley D. Sikes. “An examination of critical parameters in hybridization-based epigenotyping using magnetic microparticles,” Biotechnology Progress, 2018. DOI: 10.1002/btpr.2644.



Alan Aguirre-Soto, Kaja Kaastrup, Seunghyeon Kim, Kasite Ugo-Beke, Hadley D. Sikes. “Excitation of metastable intermediates in organic photoredox catalysis: Z-scheme approach decreases catalyst inactivation,” ACS Catalysis,” 2018. DOI:10.1021/acscatal.8b00857.




Troy F. Langford, William M. Deen, Hadley D. Sikes. “A mathematical analysis of Prx2-STAT3 disulfide exchange rate constants for a bimolecular reaction mechanism,” Free Radical Biology and Medicine, 2018, 120: 239-245.





David M. Dellal, Emma Yee, Shefali Lathwal, Hadley D. Sikes and Jose Gomez-Marquez. “A low cost plug and play photochemistry reactor,” HardwareX, 2018, 3: 1-9.






Eric A. Miller, Subha Baniya, Daniel Osorio, Yara Jabbour Al Maalouf, and Hadley D. Sikes. “Paper-based diagnostics in the antigen-depletion regime: high density immobilization of rcSso7d-cellulose binding domain fusion proteins for efficient target capture,” Biosensors and Bioelectronics, 2018, 102: 456-463.







Emma H. Yee, Shefali Lathwal, Pratik P. Shah, Hadley D. Sikes. Detection of biomarkers of periodontal disease in human saliva using stabilized, vertical flow immunoassays,” ACS Sensors, 2017, 2 (11): 1589–1593. DOI: 10.1021/acssensors.7b00745.




Hadley D. Sikes.Redox regulation: Scaffolding H2O2 signaling,”  Nature Chemical Biology, 2017, 13: 818–819.  doi:10.1038/nchembio.2432.





Brandon W. Heimer, Brooke Tam, Alissa F. Minkovsky and Hadley D. Sikes. “Using nanobiotechnology to increase the prevalence of epigenotyping assays in precision medicine,” WIREs Nanomedicine & Nanobiotechnology, 2017, 9 (1). DOI: 10.1002/wnan.1407.






Brooke E. Tam, Ki-Joo Sung and Hadley D. Sikes. “Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA,”Molecular Systems Design and Engineering, 2016, 1: 273-277. DOI: 10.1039/C6ME00073H.





Beijing K. Huang, Kassi T. Stein and Hadley D. Sikes. “Modulating and measuring intracellular H2O2 using genetically-encoded tools to study its toxicity to human cells,” ACS Synthetic Biology, 2016, 5 (12): 1389–1395. DOI: 10.1021/acssynbio.6b00120.




Tatyana A. Shatova, Shefali Lathwal, Marissa R. Engle, Hadley D. Sikes, and Klavs F. Jensen. “Portable, constriction-expansion blood plasma separation and polymerization-based malaria detection,” Analytical Chemistry, 2016, 88 (15): 7627–7632. DOI: 10.1021/acs.analchem.6b01355.





Eric A. Miller, Michael W. Traxylmeyer, Jacqueline Shen, and Hadley D. Sikes. “Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests,” Molecular Systems Design & Engineering, 2016, 1: 377-381. DOI: 10.1039/C6ME00032K.




Shefali Lathwal and Hadley Sikes. “Assessment of colorimetric methods in paper-based immunoassay for diagnosis of malaria,” Lab on a Chip, 2016, 16: 1374-1382. DOI:10.1039/C6LC00058D. (HOT Articles Collection)





Jisam Wong and Hadley D. Sikes. “The impact of continuous oxygen flux in a thin film photopolymerization reaction with peroxy-mediated regeneration of initiator,” Macromolecular Theory and Simulations, 2016, 25 (3): 229-237. DOI: 10.1002/mats.201500098.






Beijing K. Huang, Troy F. Langford, and Hadley D. Sikes. “Using sensors and generators of H2Oto elucidate the toxicity mechanism of piperlongumine and phenethyl isothiocyanate,” Antioxidants and Redox Signaling, 2016, 24 (16): 924-938. DOI:10.1089/ars.2015.6482.





Shefali Lathwal and Hadley Sikes. “A method for designing instrument-free, quantitative immunoassays,” Analytical Chemistry, 2016, 88 (6): 3194-3202. DOI:10.1021/acs.analchem.5b04538. 



Kaja Kaastrup and Hadley D. Sikes. “Using photo-initiated polymerization reactions to detect molecular recognition,” Chemical Society Reviews, 2016, 45: 532-545. DOI:10.1039/C5CS00205B.






Joseph B. Lim, Troy F. Langford, Beijing K. Huang, William M. Deen and Hadley D. Sikes. “A reaction diffusion model of cytosolic hydrogen peroxide,”Free Radical Biology and Medicine, 2016, 90: 85-90. DOI:10.1016/j.freeadbiomed. 2015.11.005.





Kaja Kaastrup, Alan Aguirre-Soto, Chen Wang, Christopher N. Bowman, Jeffery W. Stansbury and Hadley D. Sikes “UV-Vis/FT-NIR in-situ monitoring of visible-light induced polymerization of PEGDA hydrogels initiated by eosin/triethanolamine/O2,”Polymer Chemistry, 2016, 7: 592-602. DOI:10.1039/C5PY01528F.





Lim graphical abstract Free Radical


Joseph B. Lim, Beijing K. Huang, William M. Deen and Hadley D. Sikes. “Analysis of the lifetime and spatial localization of hydrogen peroxide generated in the cytosol using a reduced kinetic model,”Free Radical Biology and Medicine, 2015, 89: 47-53. DOI:10.1016/ j.freeradbiomed.2015.07.009.



Joseph B. Lim, Kimberly A. Barker, Kristen A. Eller, Linda Jiang, Veronica Molina, Jessica Saifee, Hadley D. Sikes. “Insights into electron leakage in the reaction cycle of cytochrome P450 BM3 revealed by kinetic modeling and mutagenesis,” Protein Science, 2015, 24 (11): 1874–1883. DOI:10.1002/pro.2793. (Cover Story)







Brandon W. Heimer, Brooke E. Tam and Hadley D. Sikes. “Characterization and Directed Evolution of a Methyl Binding Domain Protein for High-Sensitivity DNA Methylation Analysis,” Protein Engineering, Design and Selection, 2015. DOI:10.1093/protein/gzv046.








Beijing K. Huang*, Sohail Ali*, Kassi T. Stein, Hadley D. Sikes. “Interpreting heterogeneity in the response of cells expressing a fluorescent biosensor to stimulation with hydrogen peroxide,”BiophysicalJournal, 2015, 109 (10): 2148–2158. DOI:10.1016/j.bpj.2015.08.053.   





new image 1 for sikes lab

Amy Reece, Kaja Kaastrup, Hadley D. Sikes and John Oakey. “Staged Inertial Microfluidic Focusing for Complex Fluid Enrichment,”RSC Advances, 2015, 5 (66): 53857-53864. DOI:10.1039/C5RA10634F.




image 2 for sikes site

Jisam Wong, Kaja Kaastrup, Alan Aguirre-Soto and Hadley D. Sikes. “A quantitative analysis of peroxy- mediated cyclic regeneration of eosin in oxygen-rich photopolymerization conditions,” Polymer, 2015, 69: 169–177. DOI:10.1016/j.polymer.2015.05.043.




Eric Miller and Hadley D. Sikes. (invited review) “Addressing barriers to the development and adoption of rapid diagnostic tests in global health,” Nanobiomedicine, 2015, 2(6). DOI:10.5772/61114.




Investigation of dendrimers-2

Kaja Kaastrup and Hadley D. Sikes. “Investigation of dendrimers functionalized with eosin as macrophotoinitiators for polymerization-based signal amplification reactions,RSC Advances, 2015, 5 (20): 15652-15659. DOI:10.1039/C4RA14466J. 





Use of a genetically



Joseph B. Lim and Hadley D. Sikes. “Use of a genetically encoded hydrogen peroxide sensor for whole cell screening of enzyme activity,” Protein Engineering, Design and Selection, 2015, 28 (3): 79-83. DOI:10.1093/protein/gzv003.



analytical biotechHadley D. Sikes and Nicola Zamboni. “Analytical Biotechnology,” Current Opinion in Biotechnology, 2015, 31: iv–vi. DOI:10.1016/j.copbio. 2014.12.001.




Polymerization based amplification for paper immunoassays copy


Abraham K. Badu-Tawiah,* Shefali Lathwal,* Kaja Kaastrup, Mohammad Al-Sayah, Dionysios C. Christodouleas, Barbara S. Smith, George M. Whitesides,* and Hadley D. Sikes.* “Polymerization-based signal amplification for paper-based immunoassays,Lab on a Chip, 2015, 15 (3):  655-659. DOI:10.1039/C4LC01239A. (Most Downloaded in 2015 Collection)




In depth HyPer


J. B. Lim, K. A. Barker, B. K. Huang, H. D. Sikes. “In-depth characterization of the fluorescent signal of HyPer, a probe for hydrogen peroxide, in bacteria exposed to external oxidative stress,Journal of Microbiological Methods, 2014, 106: 33-39. DOI:10.1016/ j.mimet.2014.07.038.



quantifying intracellular hydrogen-1 copy


B.K. Huang and H.D. Sikes. “Quantifying intracellular hydrogen peroxide perturbations in terms of concentration,Redox Biology, 2014, 2: 955–962. DOI:10.1016/j.redox.2014.08.001. (Redox Signaling Collection)



evaluating the sensitivity epigenotyping


B.W. Heimer, T.A. Shatova, J.K. Lee, K. Kaastrup and H.D. Sikes. “Evaluating the Sensitivity of Hybridization-Based Epigenotyping using a Methyl Binding Domain Protein,Analyst, 2014, 139 (15): 3695-3701. DOI:10.1039/C4AN00667D. (Cover Story)




balancing the initiation


J.K. Lee and H.D. Sikes. “Balancing the initiation and molecular recognition capabilities of eosin macroinitiators of polymerization-based signal amplification reactions,Macromolecular Rapid Communications, 2014, 35 (10): 981-986. DOI:10.1002/marc.201400035.


Impact of dissociation



K. Kaastrup, L. Chan, H.D. Sikes. “Impact of dissociation constant on detection sensitivity of polymerization-based signal amplification reactions,Analytical Chemistry, 2013, 85 (17): 8055–8060. DOI:10.1021/ac4018988.


polymerization-based signal

K. Kaastrup and H.D. Sikes. “Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times,Lab on a Chip, 2012, 12 (20): 4055-4058. DOI:10.1039/C2LC40584A.


Systematic study of



J.K. Lee, B.W. Heimer, H.D. Sikes. “Systematic study of fluorescein-functionalized macrophotoinitiators for colorimetric bioassays,” Biomacromolecules, 2012, 13 (4): 1136–1143. DOI:10.1021/bm300037t.


Functional heterologous



M.E. Boyd, B.W. Heimer, H.D. Sikes. “Functional heterologous expression and purification of a mammalian methyl-CpG binding domain in suitable yield for DNA methylation profiling assays,” Protein Expression & Purification, 2012, 82 (2): 332-338. DOI:10.1016/j.pep.2012.01.016.



H.D. Sikes, R. Jenison, C.N. Bowman. “Antigen detection using polymerization-based amplification,” Lab on a Chip, 2009, 9 (5): 653-656. DOI:10.1039/B816198D. (Featured on the cover; highlighted in RSC Chemical Biology —Research News 2, 2009).

R.R. Hansen, H.D. Sikes, C.N. Bowman. “Visual detection of labeled oligonucleotides using visible-light-polymerization-based amplification,”Biomacromolecules, 2008, 9 (1): 355-362. DOI:10.1021/bm700672z.

H.D. Sikes, R.R. Hansen, L.M. Johnson, R. Jenison, J.W. Birks, K.L. Rowlen,  and C.N. Bowman. “Using polymeric materials to generate an amplified response to molecular recognition events,Nature Materials, 2008, 7: 52-56. DOI:10.1038/nmat2042.  (Highlighted in Science 2007 318:5852, 888, in C&EN 2007 85:46, 45, and in Trends in Biotechnology, 2008 26: 11, 581-583).

J. F. Smalley, S. B.Sachs, C.E.D. Chidsey, S. P. Dudek, H. D. Sikes, S. E. Creager, C. J. Yu, S. W. Feldberg, M.D. Newton. “Interfacial electron-transfer kinetics of ferrocene through oligophenyleneethynylene bridges attached to gold electrodes as constituents of self-assembled monolayers: Observation of a nonmonotonic distance dependence,Journal of The American Chemical Society, 2004, 126 (44): 14620-14630. DOI: 10.1021/ja047458b.

H.D. Sikes, Y. Sun, S.P. Dudek, C.E.D. Chidsey, and P. Pianetta. “Photoelectron spectroscopy to probe the mechanism of electron transfer through oligophenylenevinylene bridges,TheJournal of Physical Chemistry B,2003, 107 (5): 1170-1173. DOI: 10.1021/jp026734a.

S.P. Dudek, H.D. Sikes, C.E.D. Chidsey.  “Synthesis of ferrocenethiols containing oligo (phenylenevinylene) bridges and their characterization on gold electrodes,” Journal of The American Chemical Society, 2001, 123 (33): 8033-8038. DOI: 10.1021/ja0043340.

H.D. Sikes, J.F. Smalley, S.P. Dudek, A.R. Cook, M.D. Newton, C.E.D. Chidsey, S.W. Feldberg. “Rapid electron tunneling through oligophenylenevinylene bridges,Science, 2001, 291 (5508): 1519-1523. DOI: 10.1126/science.1055745.

H.D. Sikes, D.K. Schwartz. “Two-dimensional melting of an anisotropic crystal observed at the molecular level,Science, 1997, 278 (5343): 1604-1607. DOI: 10.1126/science.278.5343.1604.

H.D. Sikes, D.K. Schwartz. “A temperature-dependent two-dimensional condensation transition during Langmuir-Blodgett deposition,Langmuir, 1997, 13 (17): 4704-4709. DOI: 10.1021/la970346w.

J.T. Woodward, I. Doudevski, H.D. Sikes, D.K. Schwartz. “Kinetics of self-assembled monolayer growth explored via submonolayer coverage of incomplete films,” TheJournal of Physical Chemistry B, 1997, 101 (38): 7535-7541. DOI: 10.1021/jp971338r.

H.D. Sikes, J.T. Woodward, D.K. Schwartz. “Pattern formation in a substrate-induced phase transition during Langmuir-Blodgett transfer,” TheJournal of Physical Chemistry, 1996, 100 (21): 9093-9097. DOI: 10.1021/jp960663w.

Patents and patents pending

“Characterization and Directed Evolution of a Methyl Binding Domain Protein for High-Sensitivity DNA Methylation Analysis.” Provisional Patent Application # 62/183,479 filed 06/23/15 with co-inventor B.W. Heimer.
“Paper-based immunoassay with polymerization-based signal amplification”
Provisional Patent Application filed 11/2013, regularized application 11/2014 with co-inventors Abraham K. Badu-Tawiah, Shefali Lathwal, Mohammad H. Al-Sayah, Christodouleas Dionysios, and George M. Whitesides.
8,652,778  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application #60/988,563 filed 11/16/2007 with C.N. Bowman, K. Rowlen, R. Hansen, H.J. Avens, Regularized Application #12/099,560 filed 4/8/2008, examined and granted in the US 2/18/2014.
7,354,706  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application # 60/501755 filed 9/09/03 with K. Rowlen, J. Birks, C.N. Bowman. Regularized Patent Application #PCT/US2004/029733 filed 9/09/04.  Examined and granted in the US 4/08/08 and in the EU 2/10. Licensed, 2010; products brought to market, 2011.