I am developing quantitative assays and tools for a more systematic study of ROS. These assays and tools are applied in context of understanding and designing better cancer chemotherapeutics.
Troy F. Langford, Beijing K. Huang, Joseph B. Lim, Sun Jin Moon, Hadley D. Sikes. “Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe,” Nature Communications, 2018, in press.
Beijing K. Huang, Kassi T. Stein and Hadley D. Sikes. “Modulating and measuring intracellular H2O2 using genetically-encoded tools to study its toxicity to human cells,” ACS Synthetic Biology, 2016, 5 (12): 1389–1395. DOI: 10.1021/acssynbio.6b00120.
Beijing K. Huang, Troy F. Langford, and Hadley D. Sikes. “Using sensors and generators of H2O2 to elucidate the toxicity mechanism of piperlongumine and phenethyl isothiocyanate,”Antioxidants and Redox Signaling, 2016, 24 (16): 924-938. DOI:10.1089/ars.2015.6482.
Joseph B. Lim, Troy F. Langford, Beijing K. Huang, William M. Deen and Hadley D. Sikes. “A reaction diffusion model of cytosolic hydrogen peroxide,” Free Radical Biology and Medicine, 2016, 89: 85-90. DOI:10.1016/j.freeadbiomed. 2015.11.005.
Beijing K. Huang*, Sohail Ali*, Kassi T. Stein, Hadley D. Sikes. “Interpreting heterogeneity in the response of cells expressing a fluorescent biosensor to stimulation with hydrogen peroxide,” Biophysical Journal, 2015, 109 (10): 2148–2158. DOI:10.1016/j.bpj.2015.08.053.
Huang, B. K., Sikes, H. D. “Quantifying intracellular hydrogen peroxide perturbations in terms of concentration,” Redox Biology, 2014, 2: 955–962. DOI:10.1016/j.redox.2014.08.001. (Redox Signaling Collection)
Lim, J. B., Barker, K. A., Huang, B. K., Sikes, H. D. “In-depth characterization of the fluorescent signal of HyPer, a probe for hydrogen peroxide, in bacteria exposed to external oxidative stress,” Journal of Microbiological Methods, 2014, 106: 33-39. DOI:10.1016/j.mimet.2014.07.038.
Post-doc, NIST, Gaithersburg, MD