Peer-Reviewed Publications

Melody A. Morris, Carolyn E. Mills, Justin M. Paloni, Eric A. Miller, Hadley D. Sikes, Bradley D. Olsen. “High-throughput screening of streptavidin-binding proteins in self-assembled solid films for directed evolution of materials,” Nano Lett. 2023, 23, 16, 7303–7310.

Hoi Lok Cheng, Huan Jia, Sing Mei Lim, Say Yong Ng, Patthara Kongsuphol, Megan E. McBee, Hadley D. Sikes. “Development of a cellulose-based 96-well plate vertical flow pull-down assay,” Analytical Methods, 2023, 15, 3483-3489.

Allison Sheen, Yash Agarwal, Keith M. Cheah, Sarah C. Cowles, Jordan A. Stinson, Joseph R. Palmeri, Hadley D. Sikes, K. Dane Wittrup. “Tumor-localized catalases can fail to alter tumor growth and transcriptional profiles in subcutaneous syngeneic mouse tumor models,” Redox Biology, 2023, 64: 102766.

Huan Jia*Nazirulmubin Abdul Moomen*Jeanette Leong*Patthara Kongsuphol*Zhi Feng Sherman LimCarmen Sze Min PuiYuxuan TanKi-Joo Sung and Hadley D. Sikes. “Engineering thermostable affinity proteins for use in high-throughput immunoassay formats,” Sensors and Diagnostics, 2023, 2, 627-631.

Dousabel M.Y. Tay, Seunghyeon Kim, Yining Hao, Emma H. Yee, Huan Jia, Sydney M. Vleck, Makaya Chilekwa, Joel Voldman, and Hadley D. Sikes. “Accelerating the Optimization of Vertical Flow Assay Performance Guided by a Rational Systematic Model-Based Approach,” Biosensors and Bioelectronics, 2023, 114977.

Hoi Lok Cheng, Sing Mei Lim, Huan Jia, Ming Wei Chen, Say Yong Ng, Xiaohong Gao, Jyoti Somani, Sharmila Sengupta, Dousabel M.Y. Tay, Patrina W.L. Chua, Sharon Y.H. Ling, Megan E. McBee, Barnaby E. Young, Hadley D. Sikes*, Peter R. Preiser*. “Rapid Evaluation of Vaccine Booster Effectiveness against SARS-CoV-2 Variants,” Microbiology Spectrum, 2022, e02257-22.

Guinevere G. Connelly, Orville O. Kirkland, Seven Bohannon, Daniel C. Lim, Robert M. Wilson, Edward J. Richards, Dousabel M. Tay, Hyuk Jee, Riley D. Hellinger, Ngoc K. Hoang, Liang Hao, Arnav Chhabra, Carmen Martin-Alonso, Edward K.W. Tan, Angela N. Koehler, Michael B. Yaffe, Wendy B. London, Pui Y. Lee, Florian Krammer, Robert C. Bohannon, Sangeeta N. Bhatia, Hadley D. Sikes, Hojun Li. “Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer,” Cell Reports Methods, 2022, 2, (8), 100273.

Elliot Corless, Yining Hao, Huan Jia, Patthara Kongsuphol, Dousabel M.Y. Tay, Say Yong Ng, Hadley D. Sikes. “Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays,” Methods in Molecular Biology, 2022, 2491: 417-469.

Wentao Dong, Mark A. Keibler, Sun Jin Moon, Patricia Cho, Nian Liu, Christian J. Berrios, Joanne Kelleher, Hadley D. Sikes, Othon Iliopoulos, Jonathan Coloff, Matthew Van der Heiden, Gregory Stephanopoulos. “Oncogenic metabolic rewiring independent of proliferative control in human mammary epithelial cells,” bioRxiv, 2022.

Huan Jia*, Eric A. Miller, Chia Ching Chan, Say Yong Ng, Mookkan Prabakaran, Meng Tao, Ian Shen-Yi Cheong, Sing Mei Lim, Ming Wei Chen, Xiaohong Gao X, Abirami R, Megan M. McBee, Peter R. Preiser, Hadley D. Sikes, Patthara Kongsuphol*. “Development and translation of a paper-based top readout vertical flow assay for SARS-CoV-2 surveillance,” Lab on a Chip, 2022, 22 (7), 1321-1332.

Sing Mei Lim*, Hoi Lok Cheng*, Huan Jia*, Patthara Kongsuphol*, Bhuvaneshwari D/O Shanmuganathan, Ming Wei Chen, Say Yong Ng, Xiaohong Gao, Shuvan Prashant Turaga, Sascha P. Heussler, Jyoti Somani, Sharmila Sengupta, Dousabel M. Y. Tay, Megan E. McBee, Barnaby E. Young, Paul A. MacAry, Hadley D. Sikes*, Peter R. Preiser*. “Finger-stick blood test to assess post-vaccination SARS-CoV-2 neutralizing antibody response against variants,” Bioengineering and Translational Medicine, 2022, e10293.

Yining Hao*, Troy F. Langford*, Sun Jin Moon, Kristen A. Eller, Hadley D. Sikes. “Screening compound libraries for H2O2-mediated cancer therapeutics using a peroxiredoxin-based sensor,” Cell Chemical Biology, 2022, 29  (4), 625-635. e3

Seunghyeon Kim and Hadley D. Sikes. “Dual photoredox catalysis strategy for enhanced photopolymerization-based colorimetric biodetection,” ACS Applied Materials & Interfaces, 2021, 13 (48), 57962-57970.

Patthara Kongsuphol*, Huan Jia*, Hoi Lok Cheng*, Yue Gu*, Bhuvaneshwari D/O Shunmuganathan*, Ming- Wei Chen, Sing Mei Lim, Say Yong Ng, Paul Ananth Tambyah, Haziq Nasir, Xiaohong Gao, Dousabel Tay, Seunghyeon Kim, Rashi Gupta, Xinlei Qian, Mary M Kozma, Kiren Purushotorman, Megan E McBee, Paul A MacAry*, Hadley D Sikes*, Peter R Preiser*. “A rapid cellulose-based point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies,” Communications Medicine, 2021, 1 (1), 1-12.

Soo-Yeon Cho, Xun Gong, Volodymyr B Koman, Matthias Kuehne, Sun Jin Moon, Manki Son, Tedrick Thomas Salim Lew, Pavlo Gordiichuk, Xiaojia Jin, Hadley D Sikes, Michael S Strano. “Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry,” Nature communications, 2021,12 (1): 1-13.

Seunghyeon Kim, Yining Hao, Eric A. Miller, Dousabel M. Y. Tay, Emma Yee, Patthara Kongsuphol, Huan Jia, Megan McBee, Peter R. Preiser, and Hadley D. Sikes. “Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection in Human Serum,” ACS Sensors, 2021, ASAP Article.

Emma H. Yee, Seunghyeon Kim, and Hadley D. Sikes. “Experimental validation of eosin-mediated photo-redox polymerization mechanism and implications for signal amplification applications,” Polymer Chemistry, 2021, Advance Article.

Seunghyeon Kim*, Emma H. Yee*, Eric A. Miller, Yining Hao, Dousabel M. Y. Tay, Ki-Joo Sung, Huan Jia, Joseph M. Johnson, Mohsan Saeed, Charles R. Mace, Deniz Yüksel Yurt and Hadley D. Sikes. “Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins,” ACS Appl. Mater. Interfaces, 2021,13 (33): 38990–39002.

Seugnhyeon Kim, Alejandra Martinez Dibildox, Alan Aguirre-Soto, and Hadley D. Sikes.“Exponential Amplification Using Photoredox Autocatalysis,” J. Am. Chem. Soc., 2021, 143 (30): 11544–11553.

Daniela Cavazos-Elizondo, Ki-Joo Sung, Sangita Vasikaran, Alan Aguirre-Soto, and Hadley D. Sikes. “Functional comparison of bioactive cellulose materials incorporating engineered binding proteins,” ACS Appl. Bio Materials, 2021, in press.

Peter L. Herzog, E. Borghi, Michael W. Traxlmayr, C. Obinger, Hadley D. Sikes, Clemens K. Peterbauer. “Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1,” Protein Engineering, Design and Selection, 2020, 33. DOI: 10.1093/protein/gzaa019.

Sun Jin Moon, Wentao Dong, Gregory N. Stephanopoulos, Hadley D. Sikes. “Oxidative pentose phosphate pathway and glucose anaplerosis support maintenance of mitochondrial NADPH pool under mitochondrial oxidative stress,” Bioengineering and Translational Medicine, 2020, 5 (3). DOI: 10.1002/btm2.10184.

Kassi T. Stein, Sun Jin Moon, Athena Nguyen, Hadley D. Sikes. “Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells,PLOS Computational Biology, 2020, 16 (9). DOI: 101317/journal.pcbi.1008202.

James F Powers, Brent Cochran, James D Baleja, Hadley D Sikes, Andrew D Pattison, Xue Zhang, Inna Lomakin, Annette Shepard-Barry, Karel Pacak, Sun Jin Moon, Troy F Langford, Kassi T Stein, Richard W Tothill, Yingbin Ouyang and Arthur S Tischler. “A xenograft and cell line model of SDH-deficient pheochromocytoma derived from Sdhb+/− rats.” Endocrine-Related Cancer,” 2020, 27 (6): 337–354. DOI: 10.1530/ERC-19-0474



Ki-Joo Sung, Yara Jabbour Al Maalouf, Quinlan R. Johns, Eric A. Miller and Hadley D. Sikes. “Binding performance of engineered rcSso7d affinity reagents versus commercial antibodies against Zika virus NS1,” Analyst, 2020, 145 (7): 2515-2519. DOI: 10.1039/D0AN00299B.






Seunghyeon Kim and Hadley D. Sikes. “Radical Polymerization Reactions for Amplified Biodetection Signals,”  Polymer Chemistry, 2020, 11 (8): 1424-1444. DOI: 10.1039/C9PY01801H.





Emma H. Yee and Hadley D. Sikes. Polymerization-based amplification for target-specific colorimetric detection of amplified Mycobacterium tuberculosis DNA on cellulose,” ACS Sensors, 2020, 5 (2): 308–312. DOI: 10.1021/acssensors.9b02424.



Eric A. Miller,* Ki-Joo Sung,* Patthara Kongsuphol, Subha Baniya, Hui Qi Aw-Yong, Vivian Tay, Yuxuan Tan, Farah M. Kabir, Karl Pang-Yeo, Isabel G . Kaspriskie, Hadley D. Sikes. “Beyond Epitope Binning: Directed in Vitro Selection of Complementary Pairs of Binding Proteins,” ACS Combinatorial Science, 2020, 22 (1): 49-60. 10.1021/acscombsci.9b00176. ACS Editors’ Choice.




Hadley D. Sikes. “Quantification of H2O2 in cells: methods and significance,” Oxidative Stress: Eustress and Distress (Sies, H., ed.), Academic Press, San Diego, 2020, 113-124. DOI: 10.1016/B978-0-12-818606-0.00008-0.





Carmen Tobos*, Seunghyeon Kim*, David Rissin, Joseph Johnson, Scott Douglas, Susan Yan, Shuai Nie, Bradley Rice, Ki-Joo Sung, Hadley Sikes, David Duffy. “Sensitivity and Binding Kinetics of an Ultra-Sensitive Chemiluminescent Enzyme-Linked Immunosorbent Assay at Arrays of Antibodies,” Journal of Immunological Methods, 2019, 147. DOI: 10.1016/j.jim.2019.112643.

*these authors contributed equally




Michael A. Lee, Carlos M. Duarte, Víctor M. Eguíluz, Daniel A. Heller, Robert Langer, Mark G. Meekan, Hadley D. Sikes, Mani Srivastava, Michael S. Strano*, Rory P. Wilson. “Can Fish and Cell Phones Teach Us about Our Health?” ACS Sensors, 2019, 4 (10): 2566-2570. DOI: 10.1021/acssensors.9b00947.





Seunghyeon Kim and Hadley D. Sikes. “Liposome-enhanced polymerization-based signal amplification for highly sensitive naked-eye biodetection in paper-based sensors,” ACSApplied Materials and Interfaces, 2019, 11 (31): 28469-28477. DOI: 10.1021/acsami.9b08125.






Qifan Zhang, Lukas Zeininger, Ki-Joo Sung, Eric A Miller, Kosuke Yoshinaga, Hadley D. Sikes, and Timothy M. Swager. “Emulsion Agglutination Assay for the Detection of Protein-Protein Interactions: An Optical Sensor for Zika Virus,” ACS Sensors, 2019, 4 (1): 180–184. DOI: 10.1021/acssensors.8b01202.






Alan Aguirre-Soto, Seunghyeon Kim, Kaja Kaastrup and Hadley Sikes. “On the role of N-vinylpyrrolidone in the aqueous radical-initiated copolymerization with PEGDA mediated by eosin Y in presence of O2,” Polymer Chemistry, 2019. DOI: 10.1039/C8PY0149K.






Ki Joo Sung, Eric A. Miller and Hadley D. Sikes. “Engineering hyperthermostable rcSso7d as reporter molecule for in vitro diagnostic tests,” Molecular Systems Design & Engineering, 2018, 3: 877-882. DOI: 10.1039/C8ME00049B.





Kassi T. Stein, Sun Jin Moon, and Hadley D. Sikes. “Mitochondrial H2O2 generation using a tunable chemogenetic tool to perturb redox homeostasis in human cells and induce cell death,” ACS Synthetic Biology, 2018. DOI: 10.1021/acssynbio.8b00174.





Justin M. Paloni, Eric A. Miller, Hadley D. Sikes, and Bradley D. Olsen. “Improved ordering in low molecular weight protein−polymer conjugates through oligomerization of the protein block,” Biomacromolecules, 2018.  DOI: 10.1021/acs.biomac.8b00928.






Seunghyeon Kim and Hadley D. Sikes. “Phenolphthalein-conjugated hydrogel formation under visible light irradiation for reducing variability of colorimetric biodetection,” ACS Applied Bio Materials, 2018. DOI: 10.1021/acsabm.8b00148.





James F. Powers, Brent Cochran, James D. Baleja, Hadley D. Sikes, Xue Zhang, Inna Lomakin, Troy F.Langford, Kassi T. Stein, Arthur S. Tischler. “A unique model for SDH- deficient GIST: an endocrine related cancer,” Endocrine-Related Cancer, 2018. DOI: 10.1530/ERC-18-0115.




Troy F. Langford, Beijing K. Huang, Joseph B. Lim, Sun Jin Moon, Hadley D. Sikes. “Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe,” Nature Communications, 2018. DOI: 10.1038/s41467-018-05557-y.




Eric A. Miller, Yara Jabbour Al Maalouf, Hadley D. Sikes. Design principles for enhancing sensitivity in paper-based diagnostics via large-volume processing,” Analytical Chemistry, 2018. DOI: 10.1021/acs.analchem.8b02113.





Brooke E. Tam, Yining Hao, Hadley D. Sikes. “An examination of critical parameters in hybridization-based epigenotyping using magnetic microparticles,” Biotechnology Progress, 2018. DOI: 10.1002/btpr.2644.





Alan Aguirre-Soto, Kaja Kaastrup, Seunghyeon Kim, Kasite Ugo-Beke, Hadley D. Sikes. “Excitation of metastable intermediates in organic photoredox catalysis: Z-scheme approach decreases catalyst inactivation,” ACS Catalysis,” 2018. DOI:10.1021/acscatal.8b00857.




Troy F. Langford, William M. Deen, Hadley D. Sikes. “A mathematical analysis of Prx2-STAT3 disulfide exchange rate constants for a bimolecular reaction mechanism,” Free Radical Biology and Medicine, 2018, 120: 239-245. DOI: 10.1016/j.freeradbiomed.2018.03.039.





David M. Dellal, Emma Yee, Shefali Lathwal, Hadley D. Sikes and Jose Gomez-Marquez. “A low cost plug and play photochemistry reactor,” HardwareX, 2018, 3: 1-9. DOI: 10.1016/j.ohx.2017.11.001.






Eric A. Miller, Subha Baniya, Daniel Osorio, Yara Jabbour Al Maalouf, and Hadley D. Sikes. “Paper-based diagnostics in the antigen-depletion regime: high density immobilization of rcSso7d-cellulose binding domain fusion proteins for efficient target capture,” Biosensors and Bioelectronics, 2018, 102: 456-463. DOI: 10.1016/j.bios.2017.11.050.






Emma H. Yee, Shefali Lathwal, Pratik P. Shah, Hadley D. Sikes. Detection of biomarkers of periodontal disease in human saliva using stabilized, vertical flow immunoassays,” ACS Sensors, 2017, 2 (11): 1589–1593. DOI: 10.1021/acssensors.7b00745.




Hadley D. Sikes. Redox regulation: Scaffolding H2O2 signaling,”  Nature Chemical Biology, 2017, 13: 818–819.  DOI:10.1038/nchembio.2432.




Brandon W. Heimer, Brooke Tam, Alissa F. Minkovsky and Hadley D. Sikes. “Using nanobiotechnology to increase the prevalence of epigenotyping assays in precision medicine,” WIREs Nanomedicine & Nanobiotechnology, 2017, 9 (1). DOI: 10.1002/wnan.1407.





Brooke E. Tam, Ki-Joo Sung and Hadley D. Sikes. “Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA,”Molecular Systems Design and Engineering, 2016, 1: 273-277. DOI: 10.1039/C6ME00073H.





Beijing K. Huang, Kassi T. Stein and Hadley D. Sikes. “Modulating and measuring intracellular H2O2 using genetically-encoded tools to study its toxicity to human cells,” ACS Synthetic Biology, 2016, 5 (12): 1389–1395. DOI: 10.1021/acssynbio.6b00120.





Tatyana A. Shatova, Shefali Lathwal, Marissa R. Engle, Hadley D. Sikes, and Klavs F. Jensen. “Portable, constriction-expansion blood plasma separation and polymerization-based malaria detection,” Analytical Chemistry, 2016, 88 (15): 7627–7632. DOI: 10.1021/acs.analchem.6b01355.




Eric A. Miller, Michael W. Traxylmayr, Jacqueline Shen, and Hadley D. Sikes. “Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests,” Molecular Systems Design & Engineering, 2016, 1: 377-381. DOI: 10.1039/C6ME00032K.



Shefali Lathwal and Hadley Sikes. “Assessment of colorimetric methods in paper-based immunoassay for diagnosis of malaria,” Lab on a Chip, 2016, 16: 1374-1382. DOI:10.1039/C6LC00058D. (HOT Articles Collection)





Jisam Wong and Hadley D. Sikes. “The impact of continuous oxygen flux in a thin film photopolymerization reaction with peroxy-mediated regeneration of initiator,” Macromolecular Theory and Simulations, 2016, 25 (3): 229-237. DOI: 10.1002/mats.201500098.





Beijing K. Huang, Troy F. Langford, and Hadley D. Sikes. “Using sensors and generators of H2Oto elucidate the toxicity mechanism of piperlongumine and phenethyl isothiocyanate,” Antioxidants and Redox Signaling, 2016, 24 (16): 924-938. DOI:10.1089/ars.2015.6482.




Shefali Lathwal and Hadley Sikes. “A method for designing instrument-free, quantitative immunoassays,” Analytical Chemistry, 2016, 88 (6): 3194-3202. DOI:10.1021/acs.analchem.5b04538. 





Kaja Kaastrup and Hadley D. Sikes. “Using photo-initiated polymerization reactions to detect molecular recognition,” Chemical Society Reviews, 2016, 45: 532-545. DOI:10.1039/C5CS00205B.






Joseph B. Lim, Troy F. Langford, Beijing K. Huang, William M. Deen and Hadley D. Sikes. “A reaction diffusion model of cytosolic hydrogen peroxide,”Free Radical Biology and Medicine, 2016, 90: 85-90. DOI:10.1016/j.freeadbiomed. 2015.11.005.





Kaja Kaastrup, Alan Aguirre-Soto, Chen Wang, Christopher N. Bowman, Jeffery W. Stansbury and Hadley D. Sikes “UV-Vis/FT-NIR in-situ monitoring of visible-light induced polymerization of PEGDA hydrogels initiated by eosin/triethanolamine/O2,”Polymer Chemistry, 2016, 7: 592-602. DOI:10.1039/C5PY01528F.




Lim graphical abstract Free Radical


Joseph B. Lim, Beijing K. Huang, William M. Deen and Hadley D. Sikes. “Analysis of the lifetime and spatial localization of hydrogen peroxide generated in the cytosol using a reduced kinetic model,”Free Radical Biology and Medicine, 2015, 89: 47-53. DOI:10.1016/ j.freeradbiomed.2015.07.009.





Joseph B. Lim, Kimberly A. Barker, Kristen A. Eller, Linda Jiang, Veronica Molina, Jessica Saifee, Hadley D. Sikes. “Insights into electron leakage in the reaction cycle of cytochrome P450 BM3 revealed by kinetic modeling and mutagenesis,” Protein Science, 2015, 24 (11): 1874–1883. DOI:10.1002/pro.2793. (Cover Story)






Brandon W. Heimer, Brooke E. Tam and Hadley D. Sikes. “Characterization and Directed Evolution of a Methyl Binding Domain Protein for High-Sensitivity DNA Methylation Analysis,” Protein Engineering, Design and Selection, 2015. DOI:10.1093/protein/gzv046.




Beijing K. Huang*, Sohail Ali*, Kassi T. Stein, Hadley D. Sikes. “Interpreting heterogeneity in the response of cells expressing a fluorescent biosensor to stimulation with hydrogen peroxide,”BiophysicalJournal, 2015, 109 (10): 2148–2158. DOI:10.1016/j.bpj.2015.08.053.   


new image 1 for sikes lab


Amy Reece, Kaja Kaastrup, Hadley D. Sikes and John Oakey. “Staged Inertial Microfluidic Focusing for Complex Fluid Enrichment,”RSC Advances, 2015, 5 (66): 53857-53864. DOI:10.1039/C5RA10634F.



image 2 for sikes site

Jisam Wong, Kaja Kaastrup, Alan Aguirre-Soto and Hadley D. Sikes. “A quantitative analysis of peroxy- mediated cyclic regeneration of eosin in oxygen-rich photopolymerization conditions,” Polymer, 2015, 69: 169–177. DOI:10.1016/j.polymer.2015.05.043.




Eric Miller and Hadley D. Sikes. (invited review) “Addressing barriers to the development and adoption of rapid diagnostic tests in global health,” Nanobiomedicine, 2015, 2(6). DOI:10.5772/61114.



Investigation of dendrimers-2



Kaja Kaastrup and Hadley D. Sikes. “Investigation of dendrimers functionalized with eosin as macrophotoinitiators for polymerization-based signal amplification reactions,RSC Advances, 2015, 5 (20): 15652-15659. DOI:10.1039/C4RA14466J. 



Use of a genetically



Joseph B. Lim and Hadley D. Sikes. “Use of a genetically encoded hydrogen peroxide sensor for whole cell screening of enzyme activity,” Protein Engineering, Design and Selection, 2015, 28 (3): 79-83. DOI:10.1093/protein/gzv003.



analytical biotech


Hadley D. Sikes and Nicola Zamboni. “Analytical Biotechnology,” Current Opinion in Biotechnology, 2015, 31: iv–vi. DOI:10.1016/j.copbio. 2014.12.001.


Polymerization based amplification for paper immunoassays copy



Abraham K. Badu-Tawiah,* Shefali Lathwal,* Kaja Kaastrup, Mohammad Al-Sayah, Dionysios C. Christodouleas, Barbara S. Smith, George M. Whitesides,* and Hadley D. Sikes.* “Polymerization-based signal amplification for paper-based immunoassays,Lab on a Chip, 2015, 15 (3):  655-659. DOI:10.1039/C4LC01239A. (Most Downloaded in 2015 Collection)



In depth HyPer


J. B. Lim, K. A. Barker, B. K. Huang, H. D. Sikes. “In-depth characterization of the fluorescent signal of HyPer, a probe for hydrogen peroxide, in bacteria exposed to external oxidative stress,Journal of Microbiological Methods, 2014, 106: 33-39. DOI:10.1016/ j.mimet.2014.07.038.



quantifying intracellular hydrogen-1 copy


B.K. Huang and H.D. Sikes. “Quantifying intracellular hydrogen peroxide perturbations in terms of concentration,Redox Biology, 2014, 2: 955–962. DOI:10.1016/j.redox.2014.08.001. (Redox Signaling Collection)



evaluating the sensitivity epigenotyping



B.W. Heimer, T.A. Shatova, J.K. Lee, K. Kaastrup and H.D. Sikes. “Evaluating the Sensitivity of Hybridization-Based Epigenotyping using a Methyl Binding Domain Protein,Analyst, 2014, 139 (15): 3695-3701. DOI:10.1039/C4AN00667D. (Cover Story)



balancing the initiation


J.K. Lee and H.D. Sikes. “Balancing the initiation and molecular recognition capabilities of eosin macroinitiators of polymerization-based signal amplification reactions,Macromolecular Rapid Communications, 2014, 35 (10): 981-986. DOI:10.1002/marc.201400035.


Impact of dissociation


K. Kaastrup, L. Chan, H.D. Sikes. “Impact of dissociation constant on detection sensitivity of polymerization-based signal amplification reactions,Analytical Chemistry, 2013, 85 (17): 8055–8060. DOI:10.1021/ac4018988.



polymerization-based signal


K. Kaastrup and H.D. Sikes. “Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times,Lab on a Chip, 2012, 12 (20): 4055-4058. DOI:10.1039/C2LC40584A.


Systematic study of


J.K. Lee, B.W. Heimer, H.D. Sikes. “Systematic study of fluorescein-functionalized macrophotoinitiators for colorimetric bioassays,” Biomacromolecules, 2012, 13 (4): 1136–1143. DOI:10.1021/bm300037t.



Functional heterologous



M.E. Boyd, B.W. Heimer, H.D. Sikes. “Functional heterologous expression and purification of a mammalian methyl-CpG binding domain in suitable yield for DNA methylation profiling assays,” Protein Expression & Purification, 2012, 82 (2): 332-338. DOI:10.1016/j.pep.2012.01.016.



H.D. Sikes, R. Jenison, C.N. Bowman. “Antigen detection using polymerization-based amplification,” Lab on a Chip, 2009, 9 (5): 653-656. DOI:10.1039/B816198D. (Featured on the cover; highlighted in RSC Chemical Biology —Research News 2, 2009).

R.R. Hansen, H.D. Sikes, C.N. Bowman. “Visual detection of labeled oligonucleotides using visible-light-polymerization-based amplification,”Biomacromolecules, 2008, 9 (1): 355-362. DOI:10.1021/bm700672z.

H.D. Sikes, R.R. Hansen, L.M. Johnson, R. Jenison, J.W. Birks, K.L. Rowlen,  and C.N. Bowman. “Using polymeric materials to generate an amplified response to molecular recognition events,Nature Materials, 2008, 7: 52-56. DOI:10.1038/nmat2042.  (Highlighted in Science 2007 318:5852, 888, in C&EN 2007 85:46, 45, and in Trends in Biotechnology, 2008 26: 11, 581-583).

J. F. Smalley, S. B.Sachs, C.E.D. Chidsey, S. P. Dudek, H. D. Sikes, S. E. Creager, C. J. Yu, S. W. Feldberg, M.D. Newton. “Interfacial electron-transfer kinetics of ferrocene through oligophenyleneethynylene bridges attached to gold electrodes as constituents of self-assembled monolayers: Observation of a nonmonotonic distance dependence,Journal of The American Chemical Society, 2004, 126 (44): 14620-14630. DOI: 10.1021/ja047458b.

H.D. Sikes, Y. Sun, S.P. Dudek, C.E.D. Chidsey, and P. Pianetta. “Photoelectron spectroscopy to probe the mechanism of electron transfer through oligophenylenevinylene bridges,TheJournal of Physical Chemistry B,2003, 107 (5): 1170-1173. DOI: 10.1021/jp026734a.

S.P. Dudek, H.D. Sikes, C.E.D. Chidsey.  “Synthesis of ferrocenethiols containing oligo (phenylenevinylene) bridges and their characterization on gold electrodes,” Journal of The American Chemical Society, 2001, 123 (33): 8033-8038. DOI: 10.1021/ja0043340.

H.D. Sikes, J.F. Smalley, S.P. Dudek, A.R. Cook, M.D. Newton, C.E.D. Chidsey, S.W. Feldberg. “Rapid electron tunneling through oligophenylenevinylene bridges,Science, 2001, 291 (5508): 1519-1523. DOI: 10.1126/science.1055745.

H.D. Sikes, D.K. Schwartz. “Two-dimensional melting of an anisotropic crystal observed at the molecular level,Science, 1997, 278 (5343): 1604-1607. DOI: 10.1126/science.278.5343.1604.

H.D. Sikes, D.K. Schwartz. “A temperature-dependent two-dimensional condensation transition during Langmuir-Blodgett deposition,Langmuir, 1997, 13 (17): 4704-4709. DOI: 10.1021/la970346w.

J.T. Woodward, I. Doudevski, H.D. Sikes, D.K. Schwartz. “Kinetics of self-assembled monolayer growth explored via submonolayer coverage of incomplete films,” TheJournal of Physical Chemistry B, 1997, 101 (38): 7535-7541. DOI: 10.1021/jp971338r.

H.D. Sikes, J.T. Woodward, D.K. Schwartz. “Pattern formation in a substrate-induced phase transition during Langmuir-Blodgett transfer,” TheJournal of Physical Chemistry, 1996, 100 (21): 9093-9097. DOI: 10.1021/jp960663w.

Patents and patents pending

“Characterization and Directed Evolution of a Methyl Binding Domain Protein for High-Sensitivity DNA Methylation Analysis.” Provisional Patent Application # 62/183,479 filed 06/23/15 with co-inventor B.W. Heimer.
“Paper-based immunoassay with polymerization-based signal amplification”
Provisional Patent Application filed 11/2013, regularized application 11/2014 with co-inventors Abraham K. Badu-Tawiah, Shefali Lathwal, Mohammad H. Al-Sayah, Christodouleas Dionysios, and George M. Whitesides.
8,652,778  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application #60/988,563 filed 11/16/2007 with C.N. Bowman, K. Rowlen, R. Hansen, H.J. Avens, Regularized Application #12/099,560 filed 4/8/2008, examined and granted in the US 2/18/2014.
7,354,706  “Photopolymerization for amplification and detection of a molecular recognition event” Provisional Patent Application # 60/501755 filed 9/09/03 with K. Rowlen, J. Birks, C.N. Bowman. Regularized Patent Application #PCT/US2004/029733 filed 9/09/04.  Examined and granted in the US 4/08/08 and in the EU 2/10. Licensed, 2010; products brought to market, 2011.