In human cells, many signaling pathways that regulate homeostasis and apoptosis are controlled by redox reactions involving cysteine residues of proteins. Function of these proteins relies on the maintenance of controlled levels of reactive oxygen species such as hydrogen peroxide in cells. It has been suggested that higher fluxes and/or levels are present in many cancer cells, placing these cells closer to a lethal level than non-cancerous cells. My project focuses on the development of high throughput screens to identify compounds that may provide therapeutic benefit via this mechanism.
Joseph B. Lim, Kimberly A. Barker, Kristen A. Eller, Linda Jiang, Veronica Molina, Jessica Saifee, Hadley D. Sikes. “Insights into electron leakage in the reaction cycle of cytochrome P450 BM3 revealed by kinetic modeling and mutagenesis,” Protein Science, 2015, 24 (11): 1874–1883. . (Cover Story)